Journal: Frontiers in Oncology
Article Title: Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients
doi: 10.3389/fonc.2023.1073793
Figure Lengend Snippet: Bone marrow (BM) and peripheral blood (PB) spontaneously derived osteoclasts (OCs) are mature and functional. (A) Representative pictures showing cell surface antigens MMP-9, c-Src and αV integrin (CD51) identifying mature OC cultured from a breast cancer patient (BCP) on bovine cortical bone slices by immunocytochemistry. Scale bars: 100 µm. (B) Arrows show resorption lacunae acidification (acid vacuoles) on BCP derived OC by Acridine Orange staining. Acridine Orange is green at neutral pH and red at acidic pH. Scale bars: 100 µm. (C) Representative pictures showing BCP-BM derived OC resoption lacunae formation on bovine cortical slices. After OC removal, cortical bone slices were stained with Toluidine Blue; arrows indicate resoption lacunae. Scale bars: 100 µm.
Article Snippet: This step was followed by overnight 4°C primary Abs incubation: MMP-9 (mouse IgG1, MAB911, R&D Systems), c-Src kinase (mouse IgG2a, MAB3807, R&D Systems) and CD51 (goat IgG, AF1219, R&D Systems), all diluted in the blocking solution.
Techniques: Derivative Assay, Functional Assay, Cell Culture, Immunocytochemistry, Staining